Mouse macrophage β subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen
Identifieur interne : 004A62 ( Main/Exploration ); précédent : 004A61; suivant : 004A63Mouse macrophage β subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen
Auteurs : Donna L. Zeger [États-Unis] ; Narin Osman [Australie] ; Margaret Hennings [Australie] ; Ian F. C. Mckenzie [Australie] ; D. W. Sears [États-Unis] ; P. Mark Hogarth [Australie]Source :
- Immunogenetics [ 0093-7711 ] ; 1990-03-01.
English descriptors
- Teeft :
- Abundant mrna species, Acid sequence, Adhesion, Amino, Amino acid leader sequence, Amino acid sequence, Amino acids, Biol, Biol chem, Cdna, Cdna clones, Cdna inserts, Cdna libraries, Cdna sequence, Cell biol, Cell surface receptors, Clone, Coding sequence, Complement receptor, Complement receptor type, Cytoplasmic, Cytoplasmic domain, Cytoplasmic domains, Glycoprotein, Human subunits, Integrin, Kishimoto, Library screening, Lower case, Lymphocyte, Lymphocyte antigen, Macrophage, Macrophage differentiation antigen, Monoclonal antibody, Mouse fimacq subunit, Mouse integrin, Mouse sequence, Mrna, Nucleic, Nucleic acid sequence, Nucleic acid sequences, Other integrin, Overall sequence identity, Poly, Potential glycosylation sites, Primer, Probe sequence, Proc natl acad, Protein sequence, Receptor, Ruler line, Sequencing, Sequencing reactions, Springer, Subunit, Subunit cdna, Zeger.
Abstract
Abstract: The cDNA for the common \Mac-1 subunit (CD11b) of the mouse LFA-1/Mac-1/p150,95 group of leukocyte cell adhesion receptors, formally designated integrin \2, has been cloned and sequenced. Clones were isolated from cDNA libraries made from J774 macrophage and WEHI-3B myelomonocytic tumor cells which express this subunit as a component of the macrophage activation antigen 1 (Mac-1), also known as complement receptor type 3 (CR3). This subunit is expressed as a single, abundant mRNA species approximately 2.7 kilobase (kb) in size. The 2422 base pair (bp) cDNA sequence obtained codes for a 771 amino acid protein organized with leader, extracellular, transmembrane, and cytoplamic domains of 23, 680, 23, and 46 amino acids, respectively, yielding an 82700 mature protein of 747 amino acids. The mouse \Mac-1 subunit is highly similar to its human counterpart with an overall sequence identity of 81% and identical positioning of 5 out of 6 potential N-linked glycosylation sites, as well as 56 Cys residues that are organized in repeating motifs characteristic of integrin \ subunits. The most highly conserved regions are the transmembrane and cytoplasmic domains where only 4 out of 69 amino acids differ, indicating that the functions associated with this domain in Mac-1-mediated processes, such as iC3b-triggered phagocytosis, have been evolutionarily conserved.
Url:
DOI: 10.1007/BF00211555
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001938
- to stream Istex, to step Curation: 001938
- to stream Istex, to step Checkpoint: 001F87
- to stream Main, to step Merge: 004B40
- to stream Main, to step Curation: 004A62
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mouse macrophage β subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen</title><author><name sortKey="Zeger, Donna L" sort="Zeger, Donna L" uniqKey="Zeger D" first="Donna L." last="Zeger">Donna L. Zeger</name></author><author><name sortKey="Osman, Narin" sort="Osman, Narin" uniqKey="Osman N" first="Narin" last="Osman">Narin Osman</name></author><author><name sortKey="Hennings, Margaret" sort="Hennings, Margaret" uniqKey="Hennings M" first="Margaret" last="Hennings">Margaret Hennings</name></author><author><name sortKey="Mckenzie, Ian F C" sort="Mckenzie, Ian F C" uniqKey="Mckenzie I" first="Ian F. C." last="Mckenzie">Ian F. C. Mckenzie</name></author><author><name sortKey="Sears, D W" sort="Sears, D W" uniqKey="Sears D" first="D. W." last="Sears">D. W. Sears</name></author><author><name sortKey="Hogarth, P Mark" sort="Hogarth, P Mark" uniqKey="Hogarth P" first="P. Mark" last="Hogarth">P. Mark Hogarth</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:736D44A1709A99080AD16601358656CDBC9CB587</idno><date when="1990" year="1990">1990</date><idno type="doi">10.1007/BF00211555</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-PB84B3TZ-S/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001938</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001938</idno><idno type="wicri:Area/Istex/Curation">001938</idno><idno type="wicri:Area/Istex/Checkpoint">001F87</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001F87</idno><idno type="wicri:doubleKey">0093-7711:1990:Zeger D:mouse:macrophage:subunit</idno><idno type="wicri:Area/Main/Merge">004B40</idno><idno type="wicri:Area/Main/Curation">004A62</idno><idno type="wicri:Area/Main/Exploration">004A62</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Mouse macrophage β subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen</title><author><name sortKey="Zeger, Donna L" sort="Zeger, Donna L" uniqKey="Zeger D" first="Donna L." last="Zeger">Donna L. Zeger</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, Biochemistry and Molecular Biology Section, University of California, 93106, Santa Barbara, CA</wicri:regionArea><wicri:noRegion>CA</wicri:noRegion></affiliation></author><author><name sortKey="Osman, Narin" sort="Osman, Narin" uniqKey="Osman N" first="Narin" last="Osman">Narin Osman</name><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Transplantation and Cancer, University of Melbourne, 3052, Parkville, Victoria</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Hennings, Margaret" sort="Hennings, Margaret" uniqKey="Hennings M" first="Margaret" last="Hennings">Margaret Hennings</name><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Transplantation and Cancer, University of Melbourne, 3052, Parkville, Victoria</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Mckenzie, Ian F C" sort="Mckenzie, Ian F C" uniqKey="Mckenzie I" first="Ian F. C." last="Mckenzie">Ian F. C. Mckenzie</name><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Transplantation and Cancer, University of Melbourne, 3052, Parkville, Victoria</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Sears, D W" sort="Sears, D W" uniqKey="Sears D" first="D. W." last="Sears">D. W. Sears</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, Biochemistry and Molecular Biology Section, University of California, 93106, Santa Barbara, CA</wicri:regionArea><wicri:noRegion>CA</wicri:noRegion></affiliation></author><author><name sortKey="Hogarth, P Mark" sort="Hogarth, P Mark" uniqKey="Hogarth P" first="P. Mark" last="Hogarth">P. Mark Hogarth</name><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Transplantation and Cancer, University of Melbourne, 3052, Parkville, Victoria</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunogenetics</title><title level="j" type="abbrev">Immunogenetics</title><idno type="ISSN">0093-7711</idno><idno type="eISSN">1432-1211</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1990-03-01">1990-03-01</date><biblScope unit="volume">31</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="191">191</biblScope><biblScope unit="page" to="197">197</biblScope></imprint><idno type="ISSN">0093-7711</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0093-7711</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Abundant mrna species</term><term>Acid sequence</term><term>Adhesion</term><term>Amino</term><term>Amino acid leader sequence</term><term>Amino acid sequence</term><term>Amino acids</term><term>Biol</term><term>Biol chem</term><term>Cdna</term><term>Cdna clones</term><term>Cdna inserts</term><term>Cdna libraries</term><term>Cdna sequence</term><term>Cell biol</term><term>Cell surface receptors</term><term>Clone</term><term>Coding sequence</term><term>Complement receptor</term><term>Complement receptor type</term><term>Cytoplasmic</term><term>Cytoplasmic domain</term><term>Cytoplasmic domains</term><term>Glycoprotein</term><term>Human subunits</term><term>Integrin</term><term>Kishimoto</term><term>Library screening</term><term>Lower case</term><term>Lymphocyte</term><term>Lymphocyte antigen</term><term>Macrophage</term><term>Macrophage differentiation antigen</term><term>Monoclonal antibody</term><term>Mouse fimacq subunit</term><term>Mouse integrin</term><term>Mouse sequence</term><term>Mrna</term><term>Nucleic</term><term>Nucleic acid sequence</term><term>Nucleic acid sequences</term><term>Other integrin</term><term>Overall sequence identity</term><term>Poly</term><term>Potential glycosylation sites</term><term>Primer</term><term>Probe sequence</term><term>Proc natl acad</term><term>Protein sequence</term><term>Receptor</term><term>Ruler line</term><term>Sequencing</term><term>Sequencing reactions</term><term>Springer</term><term>Subunit</term><term>Subunit cdna</term><term>Zeger</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The cDNA for the common \Mac-1 subunit (CD11b) of the mouse LFA-1/Mac-1/p150,95 group of leukocyte cell adhesion receptors, formally designated integrin \2, has been cloned and sequenced. Clones were isolated from cDNA libraries made from J774 macrophage and WEHI-3B myelomonocytic tumor cells which express this subunit as a component of the macrophage activation antigen 1 (Mac-1), also known as complement receptor type 3 (CR3). This subunit is expressed as a single, abundant mRNA species approximately 2.7 kilobase (kb) in size. The 2422 base pair (bp) cDNA sequence obtained codes for a 771 amino acid protein organized with leader, extracellular, transmembrane, and cytoplamic domains of 23, 680, 23, and 46 amino acids, respectively, yielding an 82700 mature protein of 747 amino acids. The mouse \Mac-1 subunit is highly similar to its human counterpart with an overall sequence identity of 81% and identical positioning of 5 out of 6 potential N-linked glycosylation sites, as well as 56 Cys residues that are organized in repeating motifs characteristic of integrin \ subunits. The most highly conserved regions are the transmembrane and cytoplasmic domains where only 4 out of 69 amino acids differ, indicating that the functions associated with this domain in Mac-1-mediated processes, such as iC3b-triggered phagocytosis, have been evolutionarily conserved.</div></front></TEI><affiliations><list><country><li>Australie</li><li>États-Unis</li></country><region><li>Victoria (État)</li></region><settlement><li>Melbourne</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="États-Unis"><noRegion><name sortKey="Zeger, Donna L" sort="Zeger, Donna L" uniqKey="Zeger D" first="Donna L." last="Zeger">Donna L. Zeger</name></noRegion><name sortKey="Sears, D W" sort="Sears, D W" uniqKey="Sears D" first="D. W." last="Sears">D. W. Sears</name></country><country name="Australie"><region name="Victoria (État)"><name sortKey="Osman, Narin" sort="Osman, Narin" uniqKey="Osman N" first="Narin" last="Osman">Narin Osman</name></region><name sortKey="Hennings, Margaret" sort="Hennings, Margaret" uniqKey="Hennings M" first="Margaret" last="Hennings">Margaret Hennings</name><name sortKey="Hogarth, P Mark" sort="Hogarth, P Mark" uniqKey="Hogarth P" first="P. Mark" last="Hogarth">P. Mark Hogarth</name><name sortKey="Mckenzie, Ian F C" sort="Mckenzie, Ian F C" uniqKey="Mckenzie I" first="Ian F. C." last="Mckenzie">Ian F. C. Mckenzie</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004A62 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004A62 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:736D44A1709A99080AD16601358656CDBC9CB587
|texte= Mouse macrophage β subunit (CD11b) cDNA for the CR3 complement receptor/Mac-1 antigen
}}
| This area was generated with Dilib version V0.6.33. |  |